Learning from the World: Good Practices in Navigating Cultural Diversity. Bertelsmann Stiftung Study 2018

The Reinhard Mohn Prize 2018 “Living Diversity – Shaping Society” focuses on diversity in German society, that is the plurality of cultural, religious and linguistic identities found among the people who live in the country. With this focus, the RMP 2018 highlights a variety of successful strategies for living peacefully in diversity. In historical terms, cultural diversity is nothing new or unique for Germany. In fact, though we are often unaware of it, cultural diversity has been a feature of our daily life for a long time. Indeed, religious differences have shaped German society since the Reformation. And Judaism has always been present in the area we now call Germany

Soy and mustard effectively mobilize phosphorus from inorganic and organic sources

We aimed to investigate phosphorus (P) mobilization by different plant species from organic and inorganic sources in relation to different P mobilization mechanisms. Knowledge about P mobilization is important for producing crops on P sources other than phosphate rock-derived fertilizers. We conducted a greenhouse experiment with four plant species (maize, soy, lupin, mustard) and three P sources (FePO4, phytate, struvite). We determined pH and phosphomonoesterase activity in the rhizosphere using pH imaging and soil zymography. At harvest, root exudates were analyzed for phosphomonoesterase activity, pH, organic acids, and dissolved organic carbon (DOC). Plants were analyzed for biomass, root length, and P content. Struvite was more plant-available than phytate and FePO4 as indicated by higher plant P contents. Soy had the highest biomass and P content, irrespective of P source. Soy exuded up to 12.5 times more organic acids and up to 4.2 times more DOC than the other plant species. Lupin had a 122.9 times higher phosphomonoesterase activity than the other plant species with phytate. The pH in the exudate solution of mustard was on average 0.8 pH units higher than of the other plant species. P uptake by mustard and soy seemed to have also benefited from large root lengths. Taken together, our study indicates that soy has a particularly high potential to mobilize P from struvite and phytate, while mustard has a high potential to mobilize P from FePO4. Therefore, soy and mustard seem to be good options for agricultural production that relies less on phosphate rock-derived fertilizers

Mechanismen der Phosphor-Akquise von Nutzpflanzen in Mischkulturen: Die Rolle von Phosphatasen und pH-Änderungen in der Rhizosphäre

Forschungsarbeiten der letzten Jahre zeigten, dass Mischkulturen zu einer gesteigerten Phosphor (P)-Aufnahme von Nutzpflanzen und folglich zu gesteigerten Erträgen beitragen können. Die zugrundeliegenden Mechanismen der P-Akquise sind allerdings noch kaum bekannt. Ziel der Studie war es deshalb, die potentiellen Mechanismen in einem Mischkultur-Experiment zu untersuchen. Wir analysierten die pH-Änderungen und Phosphatase-Aktivitäten in der Rhizosphäre von Mais (Zea mays) in Mischkultur mit entweder Ackerbohne (Vicia faba), Blauer Süßlupine (Lupinus angustifolius) oder Weißem Senf (Sinapis alba) im Vergleich mit Mais in Monokultur. Dafür wurden die Pflanzen in Rhizoboxen kultiviert; die Phosphatase-Aktivität wurde mittels Boden-Zymographie und die pH-Änderung mithilfe von pH-Indikator-Gelen untersucht. Zusätzlich wurde mit den gleichen Pflanzen ein Feldversuch durchgeführt, um die Effekte der Nebenkulturen auf die Mais-Biomasseproduktion zu untersuchen. Erste Ergebnisse legen nahe, dass die gewählten Pflanzen unterschiedliche Mechanismen der P-Akquise nutzen (Rhizoboxen-Experiment), die mit unterschiedlichen Maiserträgen einhergehen (Feldversuch)

Plant Species Interactions in the Rhizosphere Increase Maize N and P Acquisition and Maize Yields in Intercropping

The aim of the study was to examine interspecific plant interactions that contribute to plant nitrogen (N) and phosphorus (P) acquisition and are likely the reason for overyielding in intercropping. We conducted a field and a rhizobox experiment with the same soil. Maize (Zea mays L.) was grown alone or intercropped with the companions faba bean (Vicia faba L.), soy (Glycine max (L.) Merr.), blue lupin (Lupinus angustifolius L.), or white mustard (Sinapis alba L.). We determined the isotopic N signature (delta N-15) of maize as well as soil parameters (pH, phosphatase activity, nitrate) in the field experiment. We analyzed phosphatase activities and rhizosphere pH by soil zymography and pH imaging in the rhizobox experiment. Maize N and P contents were larger in intercropping than monocropping, especially with soy and lupin in the field, indicating intercropping advantages for maize N and P acquisition. Intercropping with legumes decreased maize delta N-15 in the field, suggesting that 11-20% of maize aboveground biomass N was transferred from legumes to maize. Soil zymography revealed high phosphatase activities in the rhizosphere of lupin and faba bean. pH imaging showed a rhizosphere alkalinization by mustard, and a rhizosphere acidification by faba bean. These changes in the companions' rhizosphere likely mobilized P and were also beneficial for maize in intercropping. Taken together, our study provides evidence that the companions' ability to mobilize N and P in the rhizosphere promotes increases in maize nutrient contents and causes maize overyielding in intercropping and thus can contribute to fertilizer savings

Aktuelle Kontroversen im politiktheoretischen Diskurs: "Multikulturalismus" und "Methoden"

Review essay: 1) Heins, Volker: Der Skandal der Vielfalt: Geschichte und Konzepte des Multikulturalismus. Frankfurt am Main: Campus-Verl. 2013. ISBN 978-3-593-39969-0. 2) Zapf, Holger: Methoden der Politischen Theorie: eine Einführung. Opladen: Verlag Barbara Budrich 2013. ISBN 978-3-8474-0478-1

Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems

CRISPR/Cas9 technology is currently considered the most advanced tool for targeted genome engineering. Its sequence-dependent specificity has been explored for locus-directed transcriptional modulation. Such modulation, in particular transcriptional activation, has been proposed as key approach to overcome silencing of dormant HIV provirus in latently infected cellular reservoirs. Currently available agents for provirus activation, so-called latency reversing agents (LRAs), act indirectly through cellular pathways to induce viral transcription. However, their clinical performance remains suboptimal, possibly because reservoirs have diverse cellular identities and/or proviral DNA is intractable to the induced pathways. We have explored two CRISPR/Cas9-derived activator systems as targeted approaches to induce dormant HIV-1 proviral DNA. These systems recruit multiple transcriptional activation domains to the HIV 5'long terminal repeat (LTR), for which we have identified an optimal target region within the LTR U3 sequence. Using this target region, we demonstrate transcriptional activation of proviral genomes via the synergistic activation mediator complex in various in culture model systems for HIV latency. Observed levels of induction are comparable or indeed higher than treatment with established LRAs. Importantly, activation is complete, leading to production of infective viral particles. Our data demonstrate that CRISPR/Cas9-derived technologies can be applied to counteract HIV latency and may therefore represent promising novel approaches in the quest for HIV elimination

Climate change and management intensity alter spatial distribution and abundance of P mineralizing bacteria and arbuscular mycorrhizal fungi in mountainous grassland soils

In mountainous grasslands management adaptations are required to maintain soil functions. We investigated climate change (CC) and management effects on the abundance and potential activity of microbiota catalyzing the major steps of P transformation which are still unknown in these grasslands. Soil samples were taken from intact plant-soil mesocosms managed extensively or intensively (two vs. five mowing and slurry applications, respectively). These mesocosms were previously translocated from high to lower altitudes to simulate two CC scenarios (CC1: +1 °C warming and mean annual precipitation (MAP) of 1347 mm and CC2: +3 °C warming and MAP of 956 mm), while control mesocosms (CC0) were relocated at their original site (6 °C and MAP of 1400 mm). Specific marker genes for P-solubilization (gcd), P-mineralization (phoN, phoD, phnX and appA), P-uptake (pitA and pstS), total bacteria and arbuscular mycorrhizal fungi (AMF) were quantified by quantitative real-time PCR. Spatial distributions of phosphatase activities were analyzed in situ by zymography analysis and total organic C, N and P contents were measured. Gene abundances and enzymatic activities were comparable for both managements under CC0, except for phytase-harboring (appA) microbiota which decreased under intensive management. The abundance of microbiota which catalyzes organic P (Po) mineralization (phoN and appA) and those harboring quinoprotein glucose dehydrogenase (gcd) for P solubilization significantly dropped by interacting effects of CC2 and extensive management. The same effect was found for microbes harboring specific P transporters (pitA and pstS). Under intensive management, microbiota catalyzing Po mineralization (phoN and appA), and alkaline phosphatase activities tended to increase in CC2. Noteworthy, the AMF abundance was reduced at 0–5 cm soil depth under CC. Our results suggest that CC and extensive management reduced microbial P solubilization, mineralization and uptake, while management intensification may increase P availability, which leads to shifts in nutrient stoichiometry and decreased AMF abundance